Technical Data
031577
ACTL6B, NT (ACTL6B, ACTL6, BAF53B, Actin-like protein 6B, 53kD BRG1-associated factor B, Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B)
Description:
ACTL6B is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ACTL6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human ACTL6B.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Oma, Y., et al. Biochem. Biophys. Res. Commun. 301(2):521-528(2003)
Kuroda, Y., et al. Biochem. Biophys. Res. Commun. 299(2):328-334(2002)
Olave, I., et al. Genes Dev. 16(19):2509-2517(2002)