Technical Data
031584
ACTN3, ID (ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein)
Description:
Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ACTN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-437 amino acids from the Central region of human ACTN3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rodriguez-Romo, G., et al. Eur. J. Appl. Physiol. 110(6):1099-1106(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Berman, Y., et al. Physiology (Bethesda) 25(4):250-259(2010)
Ruiz, J.R., et al. Scand J Med Sci Sports (2010) In press :
Buxens, A., et al. Scand J Med Sci Sports (2010) In press :