Technical Data
031757
AKT1S1, CT (AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, 40kD proline-rich AKT substrate)
Description:
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
AKT1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human AKT1S1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wang, L., et al. J. Biol. Chem. 283(23):15619-15627(2008)
Fonseca, B.D., et al. Biochem. J. 411(1):141-149(2008)
Fonseca, B.D., et al. J. Biol. Chem. 282(34):24514-24524(2007)
Wang, L., et al. J. Biol. Chem. 282(27):20036-20044(2007)
Sancak, Y., et al. Mol. Cell 25(6):903-915(2007)