Technical Data
031853
AMPH, ID (AMPH, AMPH1, Amphiphysin)
Description:
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 140-168 in the Central region of human AMPH, UniProt Accession #P49418.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human AMPH.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
He, P., et al. Amino Acids 38(4):1209-1218(2010). Liu, L., et al. Protein Pept. Lett. 17(2):246-253(2010). Hou, T., et al. J. Mol. Biol. 376(4):1201-1214(2008). Zhou, P., et al. Biopolymers 90(6):792-802(2008). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004).