Technical Data
031944
AP2M1, CT (AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain, Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protei
Description:
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
AP2M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of human AP2M1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kahlfeldt, N., et al. J. Biol. Chem. 285(4):2734-2749(2010)
Boucrot, E., et al. PLoS ONE 5 (5), E10597 (2010) :
Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009)
Levecque, C., et al. Am. J. Physiol., Cell Physiol. 297 (1), C160-C168 (2009) :
Stanasila, L., et al. Mol. Pharmacol. 74(3):562-573(2008)