Technical Data
031966
APG5L (Y35) (Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP,)
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Applications:
Suitable for use in Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
APG5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y35 of human APG5L.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
Hammond E.M., et al. FEBS Lett. 425:391-395(1998)
Strausberg R.L., et al. PNAS 99:16899-16903(2002)
Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995)
Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998)
Mizushima N., et al. J. Cell Biol. 152:657-668(2001)