Technical Data
032019
ARF5, ID (ARF5, ADP-ribosylation factor 5)
Description:
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the Central region of human ARF5.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000)