Technical Data
032283
ATP5G1, ID (ATP5G1, ATP synthase lipid-binding protein, mitochondrial, ATP synthase proteolipid P1, ATPase protein 9, ATPase subunit c)
Description:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ATP5G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from the Central region of human ATP5G1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Vives-Bauza, C., et al. Mol. Biol. Cell 21(1):131-139(2010)
Wang, H.L., et al. Cytogenet. Genome Res. 109 (4), 533 (2005) :
Cross, R.L. Nature 427(6973):407-408(2004)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Wang, H., et al. Nature 396(6708):279-282(1998)