Technical Data
032286
ATP5J, ID (ATP5J, ATP5A, ATPM, ATP synthase-coupling factor 6, mitochondrial)
Description:
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the Central region of human ATP5J.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Osanai, T., et al. Cardiovasc. Res. 81(4):780-787(2009)
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Kumagai, A., et al. Atherosclerosis 200(1):45-50(2008)
Chai, S.B., et al. Circ. J. 71(5):693-697(2007)
Morava, E., et al. Am. J. Med. Genet. A 140(8):863-868(2006)