Technical Data
032291
ATP6V0A1, NT (ATP6V0A1, ATP6N1, ATP6N1A, VPP1, V-type proton ATPase 116kD subunit a isoform 1, Clathrin-coated vesicle/synaptic vesicle proton pump 116kD subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar pr
Description:
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human ATP6V0A1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Antonacopoulou, A.G., et al. Anticancer Res. 28 (2B), 1221-1227 (2008)
Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)