Technical Data
032296
ATP6V1A, ID (ATP6V1A, ATP6A1, ATP6V1A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69kD subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha)
Description:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.

Applications:
Suitable for use in Western Blot, Immunohistochemistry

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447~477 amino acids from the Central region of human ATP6V1A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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Martins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009)
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Ahmed, M., et al. J. Proteome Res. 4(3):931-940(2005)
Morel, N. Biol. Cell 95(7):453-457(2003)