Technical Data
032340
AXIN1, CT (AXIN1, AXIN, Axin-1, Axis inhibition protein 1)
Description:
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
AXIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717-746 amino acids from the C-terminal region of human AXIN1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sue Ng, S., et al. Biol. Chem. 391 (2-3), 171-180 (2010) :
Yang, L.H., et al. Mol. Cancer 9, 25 (2010) :
Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010) :
Kameoka, M., et al. AIDS Res. Hum. Retroviruses 25(10):1005-1011(2009)
Li, Q., et al. Nat. Cell Biol. 11(9):1128-1134(2009)