Technical Data
033180
CAPN3, CT (CAPN3, CANPL3, CANP3, NCL1, Calpain-3, Calcium-activated neutral proteinase 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1)
Description:
Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CAPN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610~640 amino acids from the C-terminal region of human CAPN3.
Purity:
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Charlton, R., et al. Neuromuscul. Disord. 19(7):449-457(2009)
Moretti, D., et al. Carcinogenesis 30(6):960-967(2009)
Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)
Beckmann, J.S., et al. Neuromuscul. Disord. 18(12):913-921(2008)
Shirafuji, T., et al. Rinsho Shinkeigaku 48(9):651-655(2008)
Diaz, B.G., et al. J. Biol. Chem. 279(26):27656-27666(2004)