Technical Data
033192-AP
CARM1, ID (CARM1, PRMT4, Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4) (AP)
Description:
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CARM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human CARM1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010)
Carascossa, S., et al. Genes Dev. 24(7):708-719(2010)
Kim, Y.R., et al. BMC Cancer 10, 197 (2010) :
Ito, T., et al. BMC Dev. Biol. 9, 47 (2009) :
Haiman, C.A., et al. BMC Cancer 9, 43 (2009) :