Technical Data
033256
CBX5, ID (CBX5, HP1A, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha)
Description:
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-1117 amino acids from the Central region of human CBX5.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Nozawa, R.S., et al. Nat. Cell Biol. 12(7):719-727(2010)
Zeng, W., et al. Epigenetics 5(4):287-292(2010)
Emelyanov, A.V., et al. J. Biol. Chem. 285(20):15027-15037(2010)
Kiyomitsu, T., et al. J. Cell Biol. 188(6):791-807(2010)
Chaturvedi, P., et al. PLoS ONE 5 (5), E10620 (2010) :