Technical Data
033396
CCNB3, phosphorylated (T280) (CCNB3, CYCB3, G2/mitotic-specific cyclin-B3)
Description:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chick and Drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus. Two transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Dot Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Dot blot 1:500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T280 of human CCNB3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Cheng, J., et al. Science 308(5725):1149-1154(2005)
Nguyen, T.B., et al. J. Biol. Chem. 277(44):41960-41969(2002)
Hrimech, M., et al. EMBO J. 21 (14), 3918 (2002) :
Lozano, J.C., et al. Biochem. Biophys. Res. Commun. 291(2):406-413(2002)
Mouland, A.J., et al. Virology 292(2):321-330(2002)