Technical Data
033399
CCND1, CT (T288) (CCND1, BCL1, PRAD1, G1/S-specific cyclin-D1, B Cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene)
Description:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-295 amino acids from the C-terminal region of human CCND1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aggarwal, P., et al., Cancer Cell 18(4): 329-340 (2010). 2. Iwatani, K., et al., Biochem. Biophys. Res. Commun. 400(3): 426-431 (2010). 3. Halilovic, E., et al., Cancer Res. 70(17): 6804-6814 (2010). 4. Zheng, W., et al., Anal. Quant. Cytol. Histol. 32(3): 155-160 (2010). 5. Satiroglu-Tufan, N.L., et al., Genet. Mol. Res. 9(3): 1557-1567 (2010).