Technical Data
033504
CD3EAP, NT (DNA-directed RNA polymerase I subunit RPA34, RNA polymerase I-associated factor PAF49, Anti-sense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CD3E-associated protein, CAST, A34.5, PAF49, CAST, ASE1, CD3EAP)
Description:
CD3EAP is a DNA dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF activated transcription, presumably at a step following PIC formation.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CD3EAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CD3EAP.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Yamazaki,T., et.al., J. Biol. Chem. 274 (26), 18173-18180 (1999)
Whitehead,C.M., et.al., Chromosoma 106 (8), 493-502 (1997)