Technical Data
CDC25B, ID (CDC25B, CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B)
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-51:0
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As Reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 331-360 in the central region of human CDC25B, UniProt Accession #P30305.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human CDC25B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Uchida, S., et al., Biochem. Biophys. Res. Commun. 316(1):226-232 (2004). 2. Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004). 3. Wu, W., et al., Cancer Res. 63(19):6195-6199 (2003). 4. Mils, V., et al., Exp. Cell Res. 285(1):99-106 (2003). 5. Theis-Febvre, N., et al., Oncogene 22(2):220-232 (2003).