Technical Data
033703
Cdkn1b, NT (Cdkn1b, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1)
Description:
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Mouse Cdkn1b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Cdkn1b.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Singh, A., et al. Mol. Cell. Biol. 30(21):5145-5159(2010)
Antico-Arciuch, V.G., et al. Oncogene 29(42):5678-5686(2010)
Mitsuhashi, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16331-16335(2010)
Zhang, Y., et al. Genes Dev. 24(16):1746-1757(2010)
Wang, Y.Y., et al. J Mol Cell Biol 2(4):209-216(2010)