Technical Data
CENPH, ID (CENPH, ICEN35, Centromere protein H, Interphase centromere complex protein 35)
Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:1,000
Immunohistochemistry (paraffin): 1:50-100
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
KLH-conjugated synthetic peptide mapping to a fragment somewhere within amino acids 136-162 in the central region of human CENPH.
Purified by Protein A affinity chromatography followed by peptide affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human CENPH.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.