Technical Data
033828
CHAT, NT (CHAT, Choline O-acetyltransferase)
Description:
CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.

Applications:
Suitable for use in Western Blot, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-100
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106~135 amino acids from the N-terminal region of human CHAT.
Purity:
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Shaffer, J.R., et al. Nutr Metab Cardiovasc Dis (2010) In press
Beck, M., et al. Cell Tissue Res. 338(1):37-51(2009)
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)