Technical Data
033839
CHFR, CT (CHFR, RNF196, E3 ubiquitin-protein ligase CHFR, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196)
Description:
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the C-terminal region of human CHFR.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Soutto, M., et al. Cancer 116(17):4033-4042(2010)
Kim, J.M., et al. Biochem. Biophys. Res. Commun. 395(4):515-520(2010)
Hiraki, M., et al. World J. Gastroenterol. 16(3):330-338(2010)
Baba, S., et al. Oncol. Rep. 22(5):1173-1179(2009)
Gao, Y., et al. Int. J. Biol. Markers 24(2):83-89(2009)