Technical Data
033845
CHIA, NT (CHIA, Acidic mammalian chitinase, Lung-specific protein TSA1902)
Description:
CHIA degrades chitin and chitotriose. May participate in the defense against nematodes and other pathogens. There are 3 named isoforms produced by alternative splicing. CHIA is induced via a T helper-2 (Th2)-specific, interleukin-13-mediated pathway in epithelial cells and macrophages. CHIA may be an important mediator of IL13-induced responses in Th2-dominated disorders such as asthma. CHIA hydrolysis N-acetyl-beta-D-glucosaminide 1,4-beta-linkages in chitin and chitodextrins.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CHIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CHIA.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wu, A.C., et al. J. Allergy Clin. Immunol. 125(3):754-757(2010)
Ober, C., et al. Curr Opin Allergy Clin Immunol 9(5):401-408(2009)
Seibold, M.A., et al. J. Biol. Chem. 284(29):19650-19658(2009)
Hartl, D., et al. J. Immunol. 182(8):5098-5106(2009)
Lalaker, A., et al. Am J Rhinol Allergy 23(1):8-14(2009)