Technical Data
033883-FITC
CHST2, ID (CHST2, GN6ST, Carbohydrate sulfotransferase 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, N-acetylglucosamine 6-O-sulfotransferase 1) (FITC)
Description:
N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).

Applications:
Suitable for use in Western Blot, Flow Cytometry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 309-335 in the central region of human CHST2, UniProt Accession #NP_004258.2; Q9Y4C5; Q80WV3. Species Sequence Homology: mouse
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human CHST2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) 2. Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009) 3. Desko, M.M., et al. Glycobiology 19(10):1068-1077(2009) 4. Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009) 5. Kanoh, A., et al. Glycoconj. J. 23 (5-6), 453-460 (2006)