Technical Data
033947
CLCF1, NT (CLCF1, BSF3, CLC, NNT1, Cardiotrophin-like cytokine factor 1, B Cell-stimulating factor 3, Novel neurotrophin-1)
Description:
This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CLCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 45-72 amino acids selected from the N-terminal region of human CLCF1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rousseau, F., et al. J. Biol. Chem. 283(44):30341-30350(2008)
Dagoneau, N., et al. Am. J. Hum. Genet. 80(5):966-970(2007)
Rousseau, F., et al. Proc. Natl. Acad. Sci. U.S.A. 103(26):10068-10073(2006)
Perret, D., et al. J. Biol. Chem. 279(42):43961-43970(2004)
Burger, R., et al. Br. J. Haematol. 123(5):869-878(2003)