Technical Data
CLCF1, NT (CLCF1, BSF3, CLC, NNT1, Cardiotrophin-like cytokine factor 1, B Cell-stimulating factor 3, Novel neurotrophin-1)
This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
CLCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 45-72 amino acids selected from the N-terminal region of human CLCF1.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rousseau, F., et al. J. Biol. Chem. 283(44):30341-30350(2008)
Dagoneau, N., et al. Am. J. Hum. Genet. 80(5):966-970(2007)
Rousseau, F., et al. Proc. Natl. Acad. Sci. U.S.A. 103(26):10068-10073(2006)
Perret, D., et al. J. Biol. Chem. 279(42):43961-43970(2004)
Burger, R., et al. Br. J. Haematol. 123(5):869-878(2003)