Technical Data
034120
COPS7A, CT (COPS7A, CSN7A, DERP10, COP9 signalosome complex subunit 7a, Dermal papilla-derived protein 10, JAB1-containing signalosome subunit 7a)
Description:
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
COPS7A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-274 amino acids from the C-terminal region of human COPS7A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Obuse, C., et al. Nat. Cell Biol. 6(11):1135-1141(2004)
Wolf, D.A., et al. Nat. Cell Biol. 5(12):1029-1033(2003)
Groisman, R., et al. Cell 113(3):357-367(2003)