Technical Data
034200-HRP
CRB3, ID (CRB3, Crumbs protein homolog 3) (Azide free) (HRP)
Description:
This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
CRB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the Central region of human CRB3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Labeled with HRP.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Pardossi-Piquard, R., et al. Biochemistry 46(48):13704-13710(2007)
Fan, S., et al. J. Cell Biol. 178(3):387-398(2007)
Fogg, V.C., et al. J. Cell. Sci. 118 (PT 13), 2859-2869 (2005) :
Lemmers, C., et al. Mol. Biol. Cell 15(3):1324-1333(2004)
Roh, M.H., et al. J. Cell. Sci. 116 (PT 14), 2895-2906 (2003) :