Technical Data
034229
CRISP1, CT (CRISP1, AEGL1, Cysteine-rich secretory protein 1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog)
Description:
Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. CRISP1 is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 162-191 in the C-terminal region of human CRISP1, UniProt Accession #NP_001122.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human CRISP1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mungall, A.J., et al., Nature 425(6960): 805-811 (2003). 2. Evans, J.P., Hum. Reprod. Update 8(4): 297-311 (2002). 3. Cuasnicu, P.S., et al., Arch. Med. Res. 32(6): 614-618 (2001). 4. Cohen, D.J., et al., Biol. Reprod. 65(4): 1000-1005 (2001). 5. Kirchhoff, C., Rev. Reprod. 3(2): 86-95 (1998).