Technical Data
CRYAB, ID (CRYAB, CRYA2, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component) (FITC)
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Suitable for use in Western Blot, Immunohistochemistry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
CRYAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the Central region of human CRYAB.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Jehle, S., et al. Nat. Struct. Mol. Biol. 17(9):1037-1042(2010)
Kida, E., et al. J. Neuropathol. Exp. Neurol. 69(7):745-759(2010)
Deng, Y., et al. BMB Rep 43(6):432-437(2010)
Houck, S.A., et al. PLoS ONE 5 (7), E11795 (2010) :