Technical Data
034403
CYB5A, ID (CYB5A, CYB5, Cytochrome b5, Microsomal cytochrome b5 type A)
Description:
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
0.25mg/ml
Immunogen:
CYB5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-98 amino acids from the Central region of human CYB5A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gardner, A.M., et al. J. Biol. Chem. 285(31):23850-23857(2010)
Kok, R.C., et al. J. Clin. Endocrinol. Metab. 95(3):994-999(2010)
Sacco, J.C., et al. Pharmacogenet. Genomics 20(1):26-37(2010)
Maghzal, G.J., et al. J. Biol. Chem. 283(18):12014-12025(2008)
Inui, H., et al. Biochemistry 46(35):10213-10221(2007)