Technical Data
034406
CYB5R3, CT (CYB5R3, DIA1, NADH-cytochrome b5 reductase 3, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form)
Description:
CYB5R3 encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-100

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
0.25mg/ml
Immunogen:
CYB5R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212~241 amino acids from the C-terminal region of human CYB5R3.
Purity:
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sacco, J.C., et al. Pharmacogenet. Genomics 20(1):26-37(2010)
Brajovich, M.L., et al. Immunol. Invest. 38(6):551-559(2009)
Fermo, E., et al. Blood Cells Mol. Dis. 41(1):50-55(2008)
Brandt, D.T., et al. J. Cell Biol. 178(2):193-200(2007)
Fernandez-Borja, M., et al. J. Cell. Sci. 118 (PT 12), 2661-2670 (2005)