		Technical Data
034411	CYC1, CT (CYC1, Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit)

Description:
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

0.25mg/ml

Immunogen:

CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the C-terminal region of human CYC1.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Specificity:

Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Cummings, C., et al. Apoptosis 11(7):1121-1129(2006)
Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004)
An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)
Yuan, J., et al. Biochim. Biophys. Acta 1641(1):35-41(2003)