Technical Data
034422
CYP11B2, ID (CYP11B2, Cytochrome P450 11B2, mitochondrial, Aldosterone synthase, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase)
Description:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa127-155 from the C-terminal region of human CYP11B2, conjugated to KLH (NP_000489). Cellular Location: Mitochondrion membrane
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human CYP11B2 at ~57kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, B., et al. Urology 76 (4), 1018 (2010) : Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Huriletemuer, H., et al. Neurosciences (Riyadh) 15(3):184-189(2010) 3. Cheng, X., et al. Clin. Exp. Hypertens. 32(5):301-307(2010) 4. Nelson, D.R., et al. Pharmacogenetics 14(1):1-18(2004)