Technical Data
034552
DDX28, CT (DDX28, MDDX28, Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq].

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
DDX28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the C-terminal region of human DDX28.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Valgardsdottir, R., et al. J. Biol. Chem. 278(23):21146-21154(2003)
Valgardsdottir, R., et al. J. Biol. Chem. 276(34):32056-32063(2001)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Loftus, B.J., et al. Genomics 60(3):295-308(1999)