Technical Data
034919
EDN1, CT (EDN1, Endothelin-1, Preproendothelin-1, Big endothelin-1)
Description:
The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 176-203 in the C-terminal region of human EDN1, UniProt Accession #P05305; NP_001946.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human EDN1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010) 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) 3. Feng, J., et al. Circulation 122 (11 SUPPL), S150-S155 (2010) 4. Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010) 5. Kaparianos, A., et al. Eur Rev Med Pharmacol Sci 14(8):705-719(2010)