Technical Data
EGLN3, CT (EGLN3, Egl nine homolog 3, HPH-1, Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3) (APC)
EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism (By similarity).

Suitable for use in Western Blot, Immunohistochemistry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul4C Do Not FreezeBlue IceHumanRabbit
As reported
EGLN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-231 amino acids from the C-terminal region of human EGLN3.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sato, M., et al. Exp. Cell Res. 316(17):2871-2882(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xue, J., et al. Gastroenterology 138(2):606-615(2010)
Henze, A.T., et al. Cancer Res. 70(1):357-366(2010)
Hatzimichael, E., et al. Eur. J. Haematol. 84(1):47-51(2010)