Technical Data
GABARAPL2 (F77) (GATE16, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, MAP1 light chain 3-related protein) (APC)
Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

Suitable for use in Immunohistochemistry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Immunohistochemistry: 1:10-50

Storage and Stability:
Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.
PabIgGAffinity Purified
200ul4C Do Not FreezeBlue IceHumanRabbit
As reported
GATE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68~97 amino acids from amino acid residues surrounding F77 of human GABARAPL2.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sou,Y.S., J. Biol. Chem. 281 (6), 3017-3024 (2006)
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Wiemann,S., Genome Res. 14 (10B), 2136-2144 (2004)
Sagiv,Y., EMBO J. 19 (7), 1494-1504 (2000)