Technical Data
036176
GOT1, CT (GOT1, Aspartate aminotransferase, cytoplasmic, Glutamate oxaloacetate transaminase 1, Transaminase A)
Description:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
GOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 360-390 from the C-terminal region of human GOT1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Franke, A., et al. Nat. Genet. 42(4):292-294(2010)
Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
Campos, J., et al. Eur. J. Intern. Med. 20 (3), E53-E56 (2009)