Technical Data
036205-FITC
GPLD1, ID (GPLD1, PIGPLD1, Phosphatidylinositol-glycan-specific phospholipase D, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D) (FITC)
Description:
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.

Applications:
Suitable for use in Western Blot, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
GPLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human GPLD1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Speliotes, E.K., et al. Hepatology 52(3):904-912(2010)
Tang, J.H., et al. Clin. Biochem. 42 (4-5), 400-407 (2009) :
Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008)
Wright, M.H., et al. Biochem. Biophys. Res. Commun. 369(2):478-484(2008)
Shi, M., et al. Cancer Lett. 258(2):268-275(2007)