Technical Data
HIF1A, CT (HIF1A, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8)
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 735-762 amino acids from the C-terminal region of human HIF1A.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Hindryckx, P., et al. J. Immunol. 185(10):6306-6316(2010)
Espinosa, I., et al. Am. J. Surg. Pathol. 34(11):1708-1714(2010)
Corzo, C.A., et al. J. Exp. Med. 207(11):2439-2453(2010)
Shen, G.M., et al. FEBS Lett. 584(20):4366-4372(2010)