Technical Data
037469
KIRREL, CT (KIRREL, KIRREL1, NEPH1, Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1)
Description:
NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KIRREL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human KIRREL.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)
Wagner, M.C., et al. J. Biol. Chem. 283(51):35579-35589(2008)
Hartleben, B., et al. J. Biol. Chem. 283(34):23033-23038(2008)
Harita, Y., et al. J. Biol. Chem. 283(14):9177-9186(2008)
Ihalmo, P., et al. Diabetologia 51(1):86-90(2008)