Technical Data
037738
LEP, NT (LEP, OB, OBS, Leptin, Obese protein, Obesity factor)
Description:
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Immunofluorescence: 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 8-37 in the N-terminal region of human LEP, UniProt Accession #P41159. Species Sequence Homology: mouse
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity:
Recognizes human LEP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1 Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Qin, J., et al. J. Hum. Genet. 55(10):704-706 (2010). 3. Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398 (2010). 4. Sarzynski, M.A., et al. Int J Obes (Lond) (2010). 5. Iciek, R., et al. Ginekol. Pol. 81(8):571-577 (2010)