Technical Data
LEP, NT (LEP, OB, OBS, Leptin, Obese Protein, Obesity Factor) (FITC)
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].

Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 8-37 in the N-terminal region of human LEP, UniProt Accession #P41159. Species Sequence Homology: mouse
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Recognizes human LEP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1 Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 2. Qin, J., et al. J. Hum. Genet. 55(10):704-706 (2010). 3. Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398 (2010). 4. Sarzynski, M.A., et al. Int J Obes (Lond) (2010). 5. Iciek, R., et al. Ginekol. Pol. 81(8):571-577 (2010)