Technical Data
037738-FITC
LEP, NT (LEP, OB, OBS, Leptin, Obese protein, Obesity factor) (FITC)
Description:
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human LEP.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Qin, J., et al. J. Hum. Genet. 55(10):704-706(2010)
Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398(2010)
Sarzynski, M.A., et al. Int J Obes (Lond) (2010) In press :
Iciek, R., et al. Ginekol. Pol. 81(8):571-577(2010)