Technical Data
038894
Nephrin (Y1210) (NPHS1, NPHN, Nephrin, Renal glomerulus-specific cell adhesion receptor)
Description:
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Nephrin (Y1210) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1198-1227 amino acids from human Nephrin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010)
Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010)
Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)
Aya, K., et al. Kidney Int. 57(2):401-404(2000)