Technical Data
Nephrin (Y1210) (NPHS1, NPHN, Nephrin, Renal glomerulus-specific cell adhesion receptor)
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As Reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1191-1219 of human Nephrin, UniProt Accession #O60500.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human Nephrin. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010). 3. Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010). 4. Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010). 5. Aya, K., et al. Kidney Int. 57(2):401-404(2000)