Technical Data
038894
Nephrin (Y1210) (NPHS1, NPHN, Nephrin, Renal glomerulus-specific cell adhesion receptor)
Description:
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa1191-1219 from human Nephrin (NP_004637), conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human Nephrin. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010) Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010) Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010) Aya, K., et al. Kidney Int. 57(2):401-404(2000)