Technical Data
Neutrophil elastase, NT (ELANE, ELA2, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase)
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As Reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 13-39 in the N-terminal region of human Neutrophil elastase, UniProt Accession #P08246.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human Neutrophil elastase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kallquist, L., et al. Exp. Cell Res. 316(19):3182-3196(2010) 2. Rabai, G., et al. Thromb. Res. 126 (2), E94-E101 (2010) : 3. Newburger, P.E., et al. Pediatr Blood Cancer 55(2):314-317(2010) 4. Hayashi, M., et al. J Nippon Med Sch 77(2):80-85(2010) 5. Hector, A., et al. Mediators Inflamm. 2010, 809591 (2010) :