Technical Data
PAAF1, NT (PAAF1, WDR71, Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71)
PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.

Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the N-terminal region of human PAAF1.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lassot, I., et al. Mol. Cell 25(3):369-383(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007)