Technical Data
039598
PAAF1, NT (PAAF1, WDR71, Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71)
Description:
PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the N-terminal region of human PAAF1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lassot, I., et al. Mol. Cell 25(3):369-383(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007)