Technical Data
039951
PHF5A, CT (PHF5A, PHD finger-like domain-containing protein 5A, Splicing factor 3B-associated 14kD protein)
Description:
This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes.

Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the C-terminal region of human PHF5A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)
Will, C.L., et al. RNA 10(6):929-941(2004)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)
Zhou, Z., et al. Nature 419(6903):182-185(2002)