Technical Data
041611
SF3A2, ID (SF3A2, SAP62, Splicing factor 3A subunit 2, SF3a66, Spliceosome-associated protein 62)
Description:
This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
SF3A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human SF3A2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Tanackovic, G., et al. Mol. Biol. Cell 16(3):1366-1377(2005)
Nesic, D., et al. J. Cell. Sci. 117 (PT 19), 4423-4433 (2004) :
Takenaka, K., et al. Biochem. J. 382 (PT 1), 223-230 (2004) :
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)