Technical Data
041640
SFTPB, ID (SFTPB, SFTP3, Pulmonary surfactant-associated protein B, 18kD pulmonary-surfactant protein, 6kD protein, Pulmonary surfactant-associated proteolipid SPL(Phe))
Description:
SFTPB is the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 128-158 in the central region of human SFTPB, UniProt Accession #P07988
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human SFTPB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Baekvad-Hansen, M., et al. Eur. Respir. J. (2010) In press : Kim, W.J., et al. Eur. Respir. J. (2010) In press : Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010) Cho, M.H., et al. Respir. Res. 11, 30 (2010) : Chaiworapongsa, T., et al. J. Matern. Fetal. Neonatal. Med. 21(9):663-670(2008)